Partial purification and biochemical characterization of peroxidase from rosemary (Rosmarinus officinalis L.) leaves
نویسندگان
چکیده
منابع مشابه
A comparative study of some biochemical characteristics of peroxidase isoenzymes in rosemary plant (Rosmarinus officinalis L.)
Background & Aim: Rosemary (Rosmarinus officinalis L.) includes species that have high drug and nutritional values. Considering the importance of the antioxidant enzyme system as an effective factor in increasing the resistance of plants to different environmental stresses, in this research, some biochemical properties of catalase and peroxidase enzymes of rosemary pla...
متن کاملA comparative study of some biochemical characteristics of peroxidase isoenzymes in rosemary plant (Rosmarinus officinalis L.)
Background & Aim: Rosemary (Rosmarinus officinalis L.) includes species that have high drug and nutritional values. Considering the importance of the antioxidant enzyme system as an effective factor in increasing the resistance of plants to different environmental stresses, in this research, some biochemical properties of catalase and peroxidase enzymes of rosemary pla...
متن کاملPurification and Characterization of Peroxidase from Moringa Oleifera L. Leaves
Peroxidase catalyzes the oxidation of various electron donor substrates such as phenol and aromatic amines in the presence of hydrogen peroxide. In this study, peroxidase was purified 164-fold from the leaves of Moringa oleifera L. with a recovery of 28% by ammonium sulphate precipitation, DEAE-cellulose column chromatography, Sephadex G-200 column chromatography, and Con-A column chromatograph...
متن کاملPURIFICATION AND SOME PARTIAL CHARACTERIZATION OF PEROXIDASE ISOENZYME FROM BRASSICA OLERACEA CAPITATA L.
Acetone fractionated peroxidase from crude extract of Brassica oleracea leaves (Cabbage) was purified in three steps on chromatographic columns, using Sp-Sepharose, DEAE-Sepharose and Con A-Sepharose. The specific activity of purified main isoenzyme (BOC-POD) is 1887 u/mg protein with RZ: 3.1, which is 172 times more than the RZ of crude extract with 4.3% recovery. The molecular weight of BOC-P...
متن کاملAntioxidant and antimicrobial activity of extracts obtained from rosemary (Rosmarinus officinalis) and vine (Vitis vinifera) leaves
The antioxidant properties and antimicrobial activity of rosemary extracts were compared to those of vine leaf extracts. The phenolics were quantified by HPLC. Rosemary extracts were stronger in reducing power than vine leaf extracts but possessed weaker superoxide anion radical (O2 ) scavenging capability. The antimicrobial activity was confirmed by the broth microdilution test using minimal i...
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ژورنال
عنوان ژورنال: Advanced Biomedical Research
سال: 2015
ISSN: 2277-9175
DOI: 10.4103/2277-9175.161586